FM100 Series Fluorescence Microscopy System
Product Introduction
The Fluorescence Microscope is a microscope used for observing fluorescent or phosphorescent materials. The principle involves illuminating samples with excitation light of specific wavelengths or wavelength bands. The excitation light is absorbed by fluorophores, which then emit light at longer wavelengths. Emission filters separate the emitted light from the excitation light, and the emission light illuminates the detector to achieve fluorescence imaging. In recent years, fluorescence microscopy systems have been widely applied in biological research, such as observing fluorescently labeled biomolecules, making fluorescence microscopes increasingly important.
Product Features
- Standard/Long working distance objective series (optional)
- Imaging optical path: 1X (tube lens focal length 180 mm), customizable with different magnification reducers
- Imaging path image field size: 25 mm
- Imaging path spectral range: Visible light
- Camera interface: C/M42/M52 optional
- Illumination method: Critical/Köhler illumination optional
- Illumination source: 3 W power, 365 nm wavelength LED source
- Fluorescence module: DAPI single-band UV filter (excitation 365 nm, emission 445 nm, dichroic mirror 405 nm), customizable
System Configuration and Parameters
Professional fluorescence microscopy system providing high-sensitivity imaging solutions for biomedical research and industrial inspection
System Operating Principle
The FM100 Series fluorescence microscopy system is based on fluorescence excitation and emission principles, achieving selective detection of fluorescence signals through a precise optical filter system
Excitation Light Illumination
365 nm UV LED source emits excitation light purified by the excitation filter, ensuring only specific wavelengths illuminate the sample
Fluorophore Excitation
Fluorophores in the sample absorb excitation light energy, causing electrons to transition to an excited state
Fluorescence Emission
Excited electrons return to ground state, emitting fluorescence at longer wavelengths (Stokes shift)
Signal Separation
Dichroic mirror reflects excitation light and transmits emission light, emission filter further purifies fluorescence signal
Imaging Detection
Pure fluorescence signal is magnified by objective and tube lens system before imaging on camera sensor
Fluorescence System Configuration
Excitation Light Source
- Light Type
- UV-LED
- Power
- 3 W
- Wavelength
- 365 nm
- Lifetime
- >20,000 hours
DAPI Filter Module
- Excitation
- 365 nm (bandwidth: 50 nm)
- Dichroic
- 405 nm
- Emission
- 445 nm (bandwidth: 50 nm)
- Suitable Dyes
- DAPI, Hoechst, etc.
Optical System
- Imaging Path
- 1X (180 mm tube lens)
- Image Size
- 25 mm
- Illumination
- Epi-fluorescence
- Uniformity
- >85%
Objective Series Parameters
Standard Working Distance Objective Series (45 mm parfocal distance, 200 mm tube lens focal length )
| Model | Magnification | NA | Working Distance | Focal Length | Resolution | Object Field | Image Field |
|---|---|---|---|---|---|---|---|
| Flour2.5XA | 2.5X | 0.075 | 6.2 mm | 80 mm | 4.46 µm | 10 mm | 25 mm |
| Flour5XA | 5X | 0.15 | 23.5 mm | 39 mm | 2.2 µm | 5 mm | 25 mm |
| Flour10XA | 10X | 0.3 | 22.8 mm | 20 mm | 1.1 µm | 2.5 mm | 25 mm |
| Flour20XA | 20X | 0.4 | 19.2 mm | 10 mm | 0.8 µm | 1.1 mm | 25 mm |
| Flour50XA | 50X | 0.55 | 11 mm | 4 mm | 0.6 µm | 0.44 mm | 25 mm |
Long Working Distance Objective Series (60 mm parfocal distance, 200 mm tube lens focal length )
| Model | Magnification | NA | Working Distance | Focal Length | Resolution | Object Field | Image Field |
|---|---|---|---|---|---|---|---|
| FlourL2XA | 2X | 0.055 | 33.7 mm | 100 mm | 6.1 µm | 12.5 mm | 25 mm |
| FlourL5XA | 5X | 0.14 | 33.6 mm | 40 mm | 2.2 µm | 5 mm | 25 mm |
| FlourL10XA | 10X | 0.28 | 33.4 mm | 20 mm | 1.2 µm | 2.5 mm | 25 mm |
| FlourL20XA | 20X | 0.34 | 29.5 mm | 10 mm | 0.8 µm | 1.25 mm | 25 mm |
| FlourL50XA | 50X | 0.5 | 18.9 mm | 4 mm | 0.7 µm | 0.5 mm | 25 mm |
Typical Application Cases
Professional applications of FM100 system in various fields
Biomedical Research
The FM100 Series plays a central role in biomedical fields, achieving precise identification of cellular and subcellular structures through fluorescent dye labeling techniques. The system clearly observes important structures such as cell nuclei, cytoskeleton, and cell membranes, providing critical support for cell biology research, pathological diagnosis, and drug development.
Case Parameters
- High-specificity target molecule labeling
- Precise subcellular structure localization
- Simultaneous multi-color fluorescence observation
- Live cell dynamic tracking
- Low background, high-contrast imaging
Paper Industry Quality Control
The FM100 Series is used in the paper industry to detect the distribution of fluorescent whitening agents in paper. Under 365 nm UV light excitation, bleaching agents emit bright blue fluorescence, enabling visualization of components invisible to the naked eye and providing important basis for paper quality control and formulation optimization.
- Non-destructive testing method
- Visualization of bleaching agent distribution
- Quantitative analysis of fluorescent whitening agents
- Coating uniformity evaluation
- Recycled paper component identification
Other Application Fields
Materials Science Applications
The system is used in materials science to study fluorescence characteristics of polymers, composites, and nanomaterials, enabling visual analysis of internal material structures, defect distribution, and stress states through fluorescent labeling techniques.
- Precise material defect localization
- Polymer phase separation observation
- Nanoparticle dispersion evaluation
Food Safety Testing
The FM100 Series can be used to detect natural fluorescent substances and additives in food, such as rapid screening of aflatoxins, vitamins, preservatives, providing technical support for food safety supervision.
- Rapid toxin screening
- Additive component analysis
- Microbial contamination detection
Forensic Identification
The system is used in forensics for fluorescence examination of bodily fluid stains, fibers, hair, and other evidence, achieving trace enhancement and evidence collection through specific wavelength excitation.
- Enhanced display of bodily fluid stains
- Fiber fluorescence characteristic analysis
- Fingerprint fluorescence enhancement
Fluorescence Imaging Considerations
Avoid excessive fixation during sample preparation to prevent autofluorescence
Select appropriate fluorescent dyes matching excitation/emission wavelengths
Control excitation light intensity to prevent photobleaching
Use anti-fade reagents to extend fluorescence observation time
Dark room operation to improve imaging contrast
Regular calibration of filter combinations to ensure optimal performance
Comparative Advantages Over Other Microscopy Techniques
| Comparison Technology | FM100 System Advantages |
|---|---|
| Conventional Optical Microscopy | FM100 provides extremely high detection sensitivity and specificity, capable of detecting single-molecule level fluorescence signals |
| Phase Contrast Microscopy | FM100 enables multiple labeling and molecular-specific identification, rather than relying solely on refractive index differences |
| Darkfield Microscopy | FM100 has higher signal-to-noise ratio and quantitative analysis capability, enabling fluorescence intensity quantification |
| Confocal Microscopy | FM100 system has simpler structure and lower cost, suitable for routine fluorescence observation and wide-field imaging |
System Configuration and Accessories
Standard Configuration
- FM100 main system
- 3 W 365 nm UV-LED light source
- DAPI fluorescence filter module
- Standard C-mount adapter
- UV protection goggles
Optional Accessories
- Standard working distance objective series (2.5X-50X)
- Long working distance objective series (2X-50X)
- FITC fluorescence filter module
- TRITC fluorescence filter module
- Multi-position filter turret
- M42/M52 camera adapters
- Magnification reducer system
- Critical/Köhler illumination modules
- Fluorescence reference standard slides
The FM100 Series supports customization of various fluorescence filter modules to meet observation requirements for different fluorescent dyes
FM100 System Advantages
Professional fluorescence microscopy technology achieving high-sensitivity specific imaging
Ultra-High Detection Sensitivity
Capable of detecting single-molecule level fluorescence signals, enabling visualization of extremely low concentration target substances.
Molecular-Specific Recognition
Achieves precise localization and quantitative analysis of specific molecules through fluorescent labeling technology.
Multiple Labeling Capability
Supports simultaneous observation of multiple fluorescent dyes, enabling co-localization analysis of multi-target molecules.
LED Cold Light Technology
3 W high-power UV-LED light source with low heat generation, service life exceeding 20,000 hours, and low operating costs.
Optimized Filter Design
High-quality DAPI filter module with high excitation efficiency, low background noise, providing excellent signal-to-noise ratio.
Wide-Field Imaging Advantage
Large field of view observation capability, suitable for overall imaging of large samples such as tissue sections and cell cultures.